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5The same properties also contributed to Nazi Germany’s strategy against agar’s scarcity, which — besides being supplied from Japan by submarine — relied on large pre-war stocks and on recovery methods to reuse bacteriological agar by autoclaving (boiling at around 121°C, 250°F, in a pressurized container for 30 to 60 minutes), thus liquefying and sterilizing the jelly, before purifying it again.
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This was especially vexing to physician and bacteriologist Robert Koch, who, in seeking to culture his bacteria, “bent all his power to attain the desired result by a simple and consistently successful method,” wrote bacteriologist and historian William Bulloch in his 1938 book, The History of Bacteriology. “He attempted to obtain a good medium which was at once sterile, transparent, and solid” and got some results with gelatine.6 But gelatine is easily digested by many microbes and melts at precisely the temperatures at which the disease-causing microbes Koch wanted to study grow best.
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